QPCR Study guides, Class notes & Summaries

MB (ASCP) Review & Exam Questions Part III and Answers with Complete Solutions, Graded A+ 1 / 14 1. PCR can test for what diseases?: Hep - B Herpes simplex 1 Herpes simplex 2 HIV HPV Epstein-barr Cytomegalovirus Cystic fibrosis Huntingtons Bk virus Fragile X Parovirus 2. qPCR can test for what diseases?: Herpes simplex 1 Herpes simplex 2 Epstein-barr HPV HIV Cytomegalovirus Bk virus VZV 3. RT PCR can test for what diseases?: HIV Influenza Coronavirus Ewing sarcoma Syno...

Which of the following is not a component of a nucleotide? Phosphate group Anti-codon Ribose sugar Nitrogen base - correct answers Anti-codon According to Chargaff's rule of base pairing, adenine pairs with: - correct answers Thymine What genes would be screened in a breast cancer panel? - correct answers HER2, ERBB2, BRCA1 Next Generation Sequencing uses: - correct answers Short sequence reads Purines and pyrimidines differ from each other in that: - correct answers Purines have tw...

Which PCR method has the highest specificity? A. qPCR B. Touchdown PCR C. Nested PCR D. Two-step PCR Nested PCR Given the anti-sense strand of DNA: 5'-AATTGCCGACATAGAT-3' which is the appropriate sense strand? A. 5'-ATCTATGTCGGCAATT-3' B. 5'-TTAACGGCTGTATCTA-3' C. 5'-AATTGCCGACATAGAT-3' D. 5'-GAGCACGCTATCTTAT-3' 5'-ATCTATGTCGGCAATT-3' During DNA replication, the 3' -OH of the growing DNA chain attacks which phosphate of an incoming nucleotide? A. Alpha ...

MB ASCP VERIFIED SOLUTIONS 100% GUARANTEE PASS What genes would be screened in a lung cancer panel? - ANSWER KRAS, EGFR, ALK In a retrovirus, the RNA acts as: - ANSWER Genome and mRNA To what does the AcrAB gene product create resistance to? - ANSWER Tetracycline What gene is measured following the treatment with imatinib(Gleevec)? - ANSWER BCR/Abl Blood drawn into what type of tube is compatible for genetic testing? - ANSWER K2- EDTA tube Primer dimers are due to what complimentary...

AAB Molecular Diagnostics Testing with complete solutions 2023This DNA amplification method amplifies the nucleic acid used as a probe? Ligase Chain Reaction (LCR) What enzymes are used in Ligase Chain Reactions? DNA polymerase and DNA ligase What is the function of DNA polymerase in Ligase Chain Reaction? Is used to initiate DNA polymerase. What is the key advantage of using Ligase Chain Reaction instead of Polymerase Chain Reaction? Better specificity How does L...

MB (ASCP) 2022 - Translocations, Diseases, Pharma, Polymerases, & more Chronic Myeloid Leukemia (CML) t(9;22) BCR-ABL translocation Ewing sarcoma t(11;22) EWS-FLI1 Acute Lymphocytic Leukemia (ALL) t(9;22) BCR-ABL p190 protein Follicular Lymphoma t(14;18) BCL2 translocation Mantle Cell Lymphoma t(11;14) bcl-1 translocation CCND1-IgH Burkitt Lymphoma t(8;14) MYC gene translocation Rhabdomyosarcoma (alveolar); Cancer/solid tumor t(2;13) or t(1;13) PAX3-FKHR and PAX7-FKHR "ARMS" translocatio...

1 / 14 1. PCR can test for what diseases?: Hep - B Herpes simplex 1 Herpes simplex 2 HIV HPV Epstein-barr Cytomegalovirus Cystic fibrosis Huntingtons Bk virus Fragile X Parovirus 2. qPCR can test for what diseases?: Herpes simplex 1 Herpes simplex 2 Epstein-barr HPV HIV Cytomegalovirus Bk virus VZV 3. RT PCR can test for what diseases?: HIV Influenza Coronavirus Ewing sarcoma Synovial sarcoma Hep - C 4. Multiplex PCR can test for what diseases?: B. pertussis M. pneumo...

MB (ASCP) Review & Exam Questions Part II Questions and answers 2023 What is the Beer-Lambert Law? the linear relationship between absorbance and concentration of an absorbing species. What is the Beer-Lambert Law equation? A = ebc A: absorbance e: molar absorptivity c: concentration b: path length DNA and RNA are absorbed at: 260nm Proteins are absorbed at: 280nm What is the melting temperature equation? Tm = (4 x # of GC pairs) + (2 x # of AT p...

(MB) ASCP Practice Exam Questions Answered 100% correct Which of the following is not a component of a nucleotide? Phosphate group Anti-codon Ribose sugar Nitrogen base Anti-codon According to Chargaff's rule of base pairing, adenine pairs with: Thymine What genes would be screened in a breast cancer panel? HER2, ERBB2, BRCA1 Next Generation Sequencing uses: Short sequence reads Purines and pyrimidines differ from each other in that: Purines have two rings...

ASCP MB: Extra-Practice Questions and Answers Scored A The Klenow fragment of E. coli DNA polymerase I is used: to label DNA probes by random primer labeling. What is a solution hybridization method for the detection of nucleic acid:protein interactions? gel mobility shift assay _____ involves mixing a labeled oligonucleotide with a protein extract to see if a specific interaction occurs, visible with an apparent increase in molecular size on a gel due to the protein:nucleic acid interaction. ...